Lakehead University is a public research university with campuses in Thunder Bay and Orillia, Ontario, Canada. Lakehead University, shortened to 'Lakehead U', or 'LU', is non-denominational and provincially supported. It has undergraduate programs, graduate programs, the Bora Laskin Faculty of Law, the only internationally accredited (AACSB) business school in northern Ontario, and is home to the western campus of the Northern Ontario School of Medicine.

Lakehead has more than 45,000 alumni. The main campus in Thunder Bay has about 7,900 students. As of September 2006, a new permanent extension campus in Orillia, located about 150 kilometres (93 mi) north of Toronto, has about 1,400 students.